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In Panel A, the eight circular bands represent the tracks for the different enteroaggregative E. Coli (EAEC) O104:H4 isolates sequenced in our study (from inner to outer tracks: 55989, C227-11, C734-09, C35-10, C682-09, C760-09, C754-09, and C777-09). These different bands represent coverage plots mapped against the reference genome TY2482, with blue or green indicating a high degree of coverage, yellow moderate coverage, and orange or red little or no coverage. The C227-11 coverage plot indicates near-complete sequence identity with the TY2482 genome. The annotations on the outside of the chromosome plot highlight positions of known virulence-factor genes and genes previously characterized on the AA plasmid in the E. Coli O42 strain.


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The O104:H4 isolates, including the outbreak isolates, clustered into a single, closely related clade — again, with the exception of the C35-10 isolate. The four isolates from the German outbreak (TY2482, C227-11, LB226692, and H112180280), three of which were sequenced by other groups, are particularly closely related. The Lambdalike Phage Containing the Stx2 Genes A polymerase-chain-reaction assay revealed that the outbreak isolates contained genes encoding Shiga toxin 2, a toxin that inhibits protein synthesis and causes the hemolytic–uremic syndrome.

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