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Several of the structurally divergent regions house genes that encode virulence factors. For example, C227-11 contains two lambdalike prophage elements, one of which contains the genes for Shiga toxin. The toxin-encoding phage is observed only in the outbreak isolate (, orange highlight), whereas the other prophage element (, blue highlight) is present in several additional O104:H4 isolates. Also reveals regions unique to the outbreak-strain chromosome, including structural variation in regions that include virulence-factor genes such as pic and the aai pathogenicity island (, green highlight). This particular region includes differences between the outbreak isolates and 55989 (Fig. Whole-Genome Phylogeny We used approximately 2.56 Mb of DNA, representing the conserved core genome found in 53 E. Coli and shigella genomes, including C277-11, other German outbreak strains, and the African O104:H4 strains, to generate a phylogenic tree depicting the evolution of E.
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Coli isolates, although marked diversity is also evident within this family of plasmids. Comparison of the pAA C227-11 plasmid with the prototype pAA plasmid from enteroaggregative E. Coli 042 identified only about 34,000 bp of shared sequence, and pAA C227-11 shares only 28,000 bp of the sequence with the 55989 virulence plasmid. The sequence heterogeneity among pAA plasmids in enteroaggregative E. Coli O104:H4 is much greater than that between chromosome sequences, with conservation found in core virulence features.
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In Panel A, the eight circular bands represent the tracks for the different enteroaggregative E. Coli (EAEC) O104:H4 isolates sequenced in our study (from inner to outer tracks: 55989, C227-11, C734-09, C35-10, C682-09, C760-09, C754-09, and C777-09). These different bands represent coverage plots mapped against the reference genome TY2482, with blue or green indicating a high degree of coverage, yellow moderate coverage, and orange or red little or no coverage. The C227-11 coverage plot indicates near-complete sequence identity with the TY2482 genome. The annotations on the outside of the chromosome plot highlight positions of known virulence-factor genes and genes previously characterized on the AA plasmid in the E. Coli O42 strain.
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Zune and iPod: Maximum people today look at the Zune toward the Contact, however as soon as observing how slim and amazingly minor and light it is, I think about it to be a rather exclusive hybrid that brings together traits of both equally the Contact and the Nano. It’s incredibly vibrant and gorgeous OLED display is a little bit more compact than the contact screen, still the player itself feels rather a little bit scaled-down and lighter. It weighs concerning 2/3 as significantly, and is significantly scaled-down inside of width and height, even though becoming only a hair thicker.
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