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The insertion site of the Shiga-toxin–encoding lambdalike prophage in the outbreak strain is similar to that of the Shiga-toxin–encoding lambdalike prophage in E. Coli strain EDL933. Moreover, the similarity between 933W (the Shiga-toxin–producing phage carried by EDL933) and the prophage of the outbreak strain — particularly in the regions that flank the Shiga toxin genes, which control Shiga toxin production and toxin release (Fig. 8 and 9 in the ) — suggests that production of Shiga toxin 2 by the outbreak strain may be increased by certain antibiotics. Consistent with this possibility is our finding that growth of C227-11 in medium containing ciprofloxacin (25 ng per milliliter) increased expression of the stxAB2 genes by a factor of about 80 (Fig. 10 in the ), which is similar to the elevation of Shiga toxin 2 expression reported for the enterohemorrhagic E. Coli strain EDL933 after exposure to ciprofloxacin.
Several of the structurally divergent regions house genes that encode virulence factors. For example, C227-11 contains two lambdalike prophage elements, one of which contains the genes for Shiga toxin. The toxin-encoding phage is observed only in the outbreak isolate (, orange highlight), whereas the other prophage element (, blue highlight) is present in several additional O104:H4 isolates. Also reveals regions unique to the outbreak-strain chromosome, including structural variation in regions that include virulence-factor genes such as pic and the aai pathogenicity island (, green highlight). This particular region includes differences between the outbreak isolates and 55989 (Fig. Whole-Genome Phylogeny We used approximately 2.56 Mb of DNA, representing the conserved core genome found in 53 E. Coli and shigella genomes, including C277-11, other German outbreak strains, and the African O104:H4 strains, to generate a phylogenic tree depicting the evolution of E.

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