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Panel C shows a comparison between the genome of the outbreak strain C227-11 (left semicircle) and the 55989 genome (right semicircle), mapped against the 55989 genome and the genome of the outbreak strain TY2482, respectively. The colored ribbons inside the innermost track (blue, pink, and purple) represent homologous mappings between the two genomes, C227-11 and 55989, indicating a high degree of similarity between these genomes. The red lines show the chromosomal positioning of repeat elements, such as insertion sequences and other mobile elements, which reveal more heterogeneity between the genomes than is evident from mapping regions of homology. We compared the sequence of C227-11 with the six O104:H4 strains from Africa, the previously released sequence data from outbreak-linked isolates, the available reference-genome sequences for the outbreak isolate TY2482, and the African enteroaggregative E.
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こんばんは。音楽機材を買取業者に売るケースでは、可能な限り多くの買取業者に見積もりを頼むことが、失敗せずに売る失敗しせずに手っ取り早いやり方ですね。業者の知名度アップの為にも、季節によってアンプの買取額の上乗せを実施しています。だから、キャンペーン中に出張査定を申し込めば、競合する他の楽器買取店よりより良い金額で買ってもらうことができると言えます。使わなくなった楽器を引取店に使わない音楽機材の売却するときにはマイナンバーカードなど身分証明書を見せることが求められます。身分証を見せたくないといやがる人がわりといるのですけど略奪された商品などが市場に出回るのをなくすために必要です。保証書のない楽器の売却を望むならアンプ単体で高額で買取を実施する買取業者に依頼するなど、アンプの状態を考え買取店を選べば良いでしょう。
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The 1.5-kb plasmid could be fully resolved with two reads (Fig. 1 and Table 2 in the ). One of the plasmids is similar to the pAA plasmid found in typical enteroaggregative E. Coli isolates. The pAA plasmid of C277-11 (referred to here as pAA C277-11) encodes aggregative adherence fimbriae (the AAF/I variant), the Aat complex, the dispersin protein, AggR, and other virulence factors characteristic of enteroaggregative E. Coli ( Figure 1 Comparison of Genomes of Eight Enteroaggregative Escherichia coli O104:H4 Isolates.

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The insertion site of the Shiga-toxin–encoding lambdalike prophage in the outbreak strain is similar to that of the Shiga-toxin–encoding lambdalike prophage in E. Coli strain EDL933. Moreover, the similarity between 933W (the Shiga-toxin–producing phage carried by EDL933) and the prophage of the outbreak strain — particularly in the regions that flank the Shiga toxin genes, which control Shiga toxin production and toxin release (Fig. 8 and 9 in the ) — suggests that production of Shiga toxin 2 by the outbreak strain may be increased by certain antibiotics. Consistent with this possibility is our finding that growth of C227-11 in medium containing ciprofloxacin (25 ng per milliliter) increased expression of the stxAB2 genes by a factor of about 80 (Fig. 10 in the ), which is similar to the elevation of Shiga toxin 2 expression reported for the enterohemorrhagic E. Coli strain EDL933 after exposure to ciprofloxacin.
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Coli acquiring a Shiga-toxin–encoding phage, but it is a clear case of such a strain causing a major outbreak of disease. Whether the current outbreak is due to a particularly virulent Shiga-toxin–positive enteroaggregative E. Coli, a rare epidemiologic opportunity, or both remains unclear. The similarity between the Shiga-toxin–encoding prophage in the outbreak isolate and such prophages in enterohemorrhagic E.
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Coli 55989 strain, in order to identify copy-number variations and small nucleotide variations among all the isolates (, and Table 3 and Fig. (See at NEJM.org.) The genomes of the isolates from the current outbreak (C227-11, TY2482, LB226692, and H112180280) were very similar, with only 236 differences in single-nucleotide variants detected between TY2482 and C227-11 (Table 3 and Fig. 3 in the ), suggesting that the outbreak is clonal. We observed structural variations between the C227-11 genome and the complete genome sequence of TY2482, based in part on the recently described draft assembly, that seem unlikely to be the result of misassemblies in the C227-11 genome, because these regions are identical in the C227-11 and H112180280 genomes (Fig. 4 and 5 in the ). However, we observed larger-scale deletions, insertions, inversions, and other structural variations between the O104:H4 outbreak isolates and the seven other enteroaggregative E. Coli O104:H4 isolates we sequenced ( ).
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