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DNA Preparation, Sequencing, and Comparative Analyses We purified and sequenced the genomic DNA from C227-11, from six African enteroaggregative E. Coli clinical isolates, and from five prototype enteroaggregative E. Coli isolates with the use of PacBio RS DNA sequencers (Sequence Read Archive accession number, SRA038239; GenBank accession number, AFST00000000). We also carried out a comparative analysis with the sequence of another outbreak isolate, TY2482. (See the article by Rohde et al. Elsewhere in this issue of the Journal for an analysis of sequence data obtained from TY2482. ) The provides additional details regarding DNA sequencing, assembly, resequencing analysis, and detection of DNA structural variations, as well as the construction of phylogenetic trees and the characterization of lambdalike phage elements, virulence factors, plasmids, and regions unique to the outbreak strain.
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