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Coli genomes, as well as those of 40 previously sequenced E. Coli isolates. Figure 1 Comparison of Genomes of Eight Enteroaggregative Escherichia coli O104:H4 Isolates. In Panel A, the eight circular bands represent the tracks for the different enteroaggregative E. Coli (EAEC) O104:H4 isolates sequenced in our study (from inner to outer tracks: 55989, C227-11, C734-09, C35-10, C682-09, C760-09, C754-09, and C777-09). These different bands represent coverage plots mapped against the reference genome TY2482, with blue or green indicating a high degree of coverage, yellow moderate coverage, and orange or red little or no coverage.
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Several of the structurally divergent regions house genes that encode virulence factors. For example, C227-11 contains two lambdalike prophage elements, one of which contains the genes for Shiga toxin. The toxin-encoding phage is observed only in the outbreak isolate (, orange highlight), whereas the other prophage element (, blue highlight) is present in several additional O104:H4 isolates. Also reveals regions unique to the outbreak-strain chromosome, including structural variation in regions that include virulence-factor genes such as pic and the aai pathogenicity island (, green highlight). This particular region includes differences between the outbreak isolates and 55989 (Fig. Whole-Genome Phylogeny We used approximately 2.56 Mb of DNA, representing the conserved core genome found in 53 E. Coli and shigella genomes, including C277-11, other German outbreak strains, and the African O104:H4 strains, to generate a phylogenic tree depicting the evolution of E.
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