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The O104:H4 isolates, including the outbreak isolates, clustered into a single, closely related clade — again, with the exception of the C35-10 isolate. The four isolates from the German outbreak (TY2482, C227-11, LB226692, and H112180280), three of which were sequenced by other groups, are particularly closely related. The Lambdalike Phage Containing the Stx2 Genes A polymerase-chain-reaction assay revealed that the outbreak isolates contained genes encoding Shiga toxin 2, a toxin that inhibits protein synthesis and causes the hemolytic–uremic syndrome.
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