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Coli 55989 strain, in order to identify copy-number variations and small nucleotide variations among all the isolates (, and Table 3 and Fig. (See at NEJM.org.) The genomes of the isolates from the current outbreak (C227-11, TY2482, LB226692, and H112180280) were very similar, with only 236 differences in single-nucleotide variants detected between TY2482 and C227-11 (Table 3 and Fig. 3 in the ), suggesting that the outbreak is clonal. We observed structural variations between the C227-11 genome and the complete genome sequence of TY2482, based in part on the recently described draft assembly, that seem unlikely to be the result of misassemblies in the C227-11 genome, because these regions are identical in the C227-11 and H112180280 genomes (Fig. 4 and 5 in the ). However, we observed larger-scale deletions, insertions, inversions, and other structural variations between the O104:H4 outbreak isolates and the seven other enteroaggregative E. Coli O104:H4 isolates we sequenced ( ).
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Aderaldo Luciano, revisor e cordenador editorial de alguns dos meus livretos de Cordel lançados pela editora Luzeiro em São Paulo, vem se consolidando como um dos mais “sério, confiável e completo pesquisador e defensor” do nosso Cordel. Os livretos do Cordel da Editora Luzeiro, a partir de 2010 foram mordenizados por esse mestre, apesar de opiniões contrárias as mudanças. O Cordel não é a roupagem própria de um tempo, estática, física, imutável. O Cordel é um gênero da literatura brasileira, tal o soneto e a trova. Parabéns Aderaldo.
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DNA Preparation, Sequencing, and Comparative Analyses We purified and sequenced the genomic DNA from C227-11, from six African enteroaggregative E. Coli clinical isolates, and from five prototype enteroaggregative E. Coli isolates with the use of PacBio RS DNA sequencers (Sequence Read Archive accession number, SRA038239; GenBank accession number, AFST00000000). We also carried out a comparative analysis with the sequence of another outbreak isolate, TY2482. (See the article by Rohde et al. Elsewhere in this issue of the Journal for an analysis of sequence data obtained from TY2482. ) The provides additional details regarding DNA sequencing, assembly, resequencing analysis, and detection of DNA structural variations, as well as the construction of phylogenetic trees and the characterization of lambdalike phage elements, virulence factors, plasmids, and regions unique to the outbreak strain.

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