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The pAA C227-11 plasmid encodes a number of enteroaggregative E. Coli–specific virulence factors (, and Tables 8 and 9 in the ), such as aggR and genes that it regulates, including AggR, AatPA–D, Aap, AggA–D, and SepA, and thus presumably is critical for C227-11 pathogenicity. Discussion The outbreak clone is a dramatic example of gene acquisition by means of lateral transfer that resulted in an accretion of synergistic virulence factors. By all molecular definitions, it is an enteroaggregative E. Coli strain but one that has acquired a Shiga-toxin–encoding phage — a hallmark of Shiga-toxin–producing E.

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Background A large outbreak of diarrhea and the hemolytic–uremic syndrome caused by an unusual serotype of Shiga-toxin–producing Escherichia coli (O104:H4) began in Germany in May 2011. As of July 22, a large number of cases of diarrhea caused by Shiga-toxin–producing E. Coli have been reported — 3167 without the hemolytic–uremic syndrome (16 deaths) and 908 with the hemolytic–uremic syndrome (34 deaths) — indicating that this strain is notably more virulent than most of the Shiga-toxin–producing E. Coli strains. Preliminary genetic characterization of the outbreak strain suggested that, unlike most of these strains, it should be classified within the enteroaggregative pathotype of E. Methods We used third-generation, single-molecule, real-time DNA sequencing to determine the complete genome sequence of the German outbreak strain, as well as the genome sequences of seven diarrhea-associated enteroaggregative E.


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ad delikátní problém. Ano, souhlasím s tím, že oživení zrušené právní normy je delikátní. Jenže když je právě ono zrušení protiústavní, tak nezrušením změny nebo dokonce zrušením původní normy ústavní soud aprobuje protiústavnost.Myslím, že mnohem horší než oživení je díra v zákoně. Stěžovatel, případně ÚS, si vyzobe z předpisu protiústavní normy a hle, najednou je celý předpis neaplikovatelný. Případně zruší i ústavní právní normy, jako v části V o. s. ř.

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