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This paucity of novel DNA further supports the recent emergence of the O104:H4 lineage. As depicted in, sequences absent from C227-11 relative to the reference strain 44898 are also absent from other O104:H4 isolates. Thus, our sequence analyses suggest that acquisition of stx2, perhaps enhanced by an unusual complement of SPATEs, could account for the elevated virulence of C227-11. Plasmid Sequences and Other Markers of Clinical Significance We mapped raw sequences from the 12 isolates to the three plasmid sequences assembled as part of the TY2482 outbreak genome ( ) (GenBank accession number, AFOG01000000). The largest and smallest plasmids did not contain genes encoding any known virulence factors. However, the largest plasmid, pESBL C227-11, which encodes the extended-spectrum beta-lactamase CTX-M-15, is very similar to the pEC_Bactec plasmid found in several clinical E.

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1 in the ) to assemble the C227-11 genome and obtained 33 contigs (N50 of 402 kb and a maximum contig size of 622 kb), covering 99.7% of the bacterial chromosome at a level of accuracy of 99.97% (Table 3 in the ). (A contig is a contiguous sequence of DNA with no gaps; N50 is the contig length so that all contigs of that length or greater cover 50% of the bases in the genome.) Four additional contigs covered two large plasmids (approximately 88 kb and 75 kb) and a third 1.5-kb plasmid.


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