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Our findings suggest that critical events in the evolution of the German enteroaggregative E. Coli outbreak strain included the acquisition of a Stx-encoding prophage and a plasmid bearing an extended-spectrum beta-lactamase gene by an ancestral precursor of this strain (Fig. However, analyses of the genomes of contemporary isolates cannot reveal the true complexity of the evolutionary pathway that yielded the Shiga-toxin–producing enteroaggregative E. Coli O104:H4 outbreak strain. The outbreak is not the first clinically linked instance of an enteroaggregative E.
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