1 in the ) to assemble the C227-11 genome and obtained 33 contigs (N50 of 402 kb and a maximum contig size of 622 kb), covering 99.7% of the bacterial chromosome at a level of accuracy of 99.97% (Table 3 in the ). (A contig is a contiguous sequence of DNA with no gaps; N50 is the contig length so that all contigs of that length or greater cover 50% of the bases in the genome.) Four additional contigs covered two large plasmids (approximately 88 kb and 75 kb) and a third 1.5-kb plasmid.
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Coli 55989 strain, in order to identify copy-number variations and small nucleotide variations among all the isolates (, and Table 3 and Fig. (See at NEJM.org.) The genomes of the isolates from the current outbreak (C227-11, TY2482, LB226692, and H112180280) were very similar, with only 236 differences in single-nucleotide variants detected between TY2482 and C227-11 (Table 3 and Fig. 3 in the ), suggesting that the outbreak is clonal. We observed structural variations between the C227-11 genome and the complete genome sequence of TY2482, based in part on the recently described draft assembly, that seem unlikely to be the result of misassemblies in the C227-11 genome, because these regions are identical in the C227-11 and H112180280 genomes (Fig. 4 and 5 in the ). However, we observed larger-scale deletions, insertions, inversions, and other structural variations between the O104:H4 outbreak isolates and the seven other enteroaggregative E. Coli O104:H4 isolates we sequenced ( ).
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Coli isolates, although marked diversity is also evident within this family of plasmids. Comparison of the pAA C227-11 plasmid with the prototype pAA plasmid from enteroaggregative E. Coli 042 identified only about 34,000 bp of shared sequence, and pAA C227-11 shares only 28,000 bp of the sequence with the 55989 virulence plasmid. The sequence heterogeneity among pAA plasmids in enteroaggregative E. Coli O104:H4 is much greater than that between chromosome sequences, with conservation found in core virulence features.
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While we have no issues with Indian people, but the influx of Indian labor (legally and illegally) controlling private enterprises, functioning as CEOs, holding key portfolios and functioning as “advisors” is something that needs to be addressed NOW because Sri Lanka’s army can only protect the nation but Sri Lanka’s political leaders appear to have no answers for the calamity that is likely to result by blindly allowing the Indian influx on the excuse of cultural, diplomatic and economic ties – the problem is nothing that the armed forces can solve ever.
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POKEMON GO ★Features★ It's simple and fun to play this Jewel Quest with 2 main game modes: Arcade , Classic .Create electrifying special gems like Flame ,Jewels Diamod , star gems, Hypercubes and Supernova. https://play.google.com/store/apps/details?id=com.game.jewelsdiamond ★★Arcade Mode★★: More than 300+ challenging levels and many pretty worlds in the game. ◆ Match 3 to clear the cells ◆ Match 4 can win the jewel bomb and 1 lighting. ◆ Match 5 can win color-changing jewel and 2 lightings. ◆ Specials Items ● The bomb can eliminate the jewels around. ● The energy can eliminate to any other colored. ● The timing can extend the playing time. ● Swapping and matching your way, show your best strategical moves to get highest scores to digging to mania diamond treasure.
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