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Background A large outbreak of diarrhea and the hemolytic–uremic syndrome caused by an unusual serotype of Shiga-toxin–producing Escherichia coli (O104:H4) began in Germany in May 2011. As of July 22, a large number of cases of diarrhea caused by Shiga-toxin–producing E. Coli have been reported — 3167 without the hemolytic–uremic syndrome (16 deaths) and 908 with the hemolytic–uremic syndrome (34 deaths) — indicating that this strain is notably more virulent than most of the Shiga-toxin–producing E. Coli strains. Preliminary genetic characterization of the outbreak strain suggested that, unlike most of these strains, it should be classified within the enteroaggregative pathotype of E. Methods We used third-generation, single-molecule, real-time DNA sequencing to determine the complete genome sequence of the German outbreak strain, as well as the genome sequences of seven diarrhea-associated enteroaggregative E.


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Isolates We isolated an enteroaggregative E. Coli strain, C227-11, from a 64-year-old woman from Hamburg, Germany, who was hospitalized at Hvidovre University Hospital, Copenhagen. She presented with bloody diarrhea; the hemolytic–uremic syndrome did not develop. We selected five reference isolates (JM221, 17-2, 042, 55989, and C1010-00) to represent the best-studied enteroaggregative E. Coli strains and to capture the geographic, serotype, and virulence-factor diversity of the enteroaggregative E. Coli pathotype. We cultured six O104:H4 clinical isolates that we obtained from the Statens Serum Institute, Copenhagen (C35-10, C682-09, C734-09, C754-09, C760-09, and C777-09), the five enteroaggregative E.

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