Isolates We isolated an enteroaggregative E. Coli strain, C227-11, from a 64-year-old woman from Hamburg, Germany, who was hospitalized at Hvidovre University Hospital, Copenhagen. She presented with bloody diarrhea; the hemolytic–uremic syndrome did not develop. We selected five reference isolates (JM221, 17-2, 042, 55989, and C1010-00) to represent the best-studied enteroaggregative E. Coli strains and to capture the geographic, serotype, and virulence-factor diversity of the enteroaggregative E. Coli pathotype. We cultured six O104:H4 clinical isolates that we obtained from the Statens Serum Institute, Copenhagen (C35-10, C682-09, C734-09, C754-09, C760-09, and C777-09), the five enteroaggregative E.
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