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Coli 55989 strain, in order to identify copy-number variations and small nucleotide variations among all the isolates (, and Table 3 and Fig. (See at NEJM.org.) The genomes of the isolates from the current outbreak (C227-11, TY2482, LB226692, and H112180280) were very similar, with only 236 differences in single-nucleotide variants detected between TY2482 and C227-11 (Table 3 and Fig. 3 in the ), suggesting that the outbreak is clonal. We observed structural variations between the C227-11 genome and the complete genome sequence of TY2482, based in part on the recently described draft assembly, that seem unlikely to be the result of misassemblies in the C227-11 genome, because these regions are identical in the C227-11 and H112180280 genomes (Fig. 4 and 5 in the ). However, we observed larger-scale deletions, insertions, inversions, and other structural variations between the O104:H4 outbreak isolates and the seven other enteroaggregative E. Coli O104:H4 isolates we sequenced ( ).
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