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In Panel A, the eight circular bands represent the tracks for the different enteroaggregative E. Coli (EAEC) O104:H4 isolates sequenced in our study (from inner to outer tracks: 55989, C227-11, C734-09, C35-10, C682-09, C760-09, C754-09, and C777-09). These different bands represent coverage plots mapped against the reference genome TY2482, with blue or green indicating a high degree of coverage, yellow moderate coverage, and orange or red little or no coverage. The C227-11 coverage plot indicates near-complete sequence identity with the TY2482 genome. The annotations on the outside of the chromosome plot highlight positions of known virulence-factor genes and genes previously characterized on the AA plasmid in the E. Coli O42 strain.
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The 1.5-kb plasmid could be fully resolved with two reads (Fig. 1 and Table 2 in the ). One of the plasmids is similar to the pAA plasmid found in typical enteroaggregative E. Coli isolates. The pAA plasmid of C277-11 (referred to here as pAA C277-11) encodes aggregative adherence fimbriae (the AAF/I variant), the Aat complex, the dispersin protein, AggR, and other virulence factors characteristic of enteroaggregative E. Coli ( Figure 1 Comparison of Genomes of Eight Enteroaggregative Escherichia coli O104:H4 Isolates.
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Do we need to repeat regarding the sub-standard pharmaceutical drugs flooding to Sri Lanka? Has giving oil distribution to India not resulted in India repatriating ¾ times the capital it invested? Is India not killing our Ceylon tea now that it has secured practically all our tea plantations and are mixing our teas with India low quality tea and selling them as Pure Ceylon tea, will this subquality sold cheap not end up Sri Lanka losing the proud place it held and why is Sri Lanka watching this rape of our Ceylon tea take place – where is the benefit to Sri Lanka apart from the initial investment?
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Panel C shows a comparison between the genome of the outbreak strain C227-11 (left semicircle) and the 55989 genome (right semicircle), mapped against the 55989 genome and the genome of the outbreak strain TY2482, respectively. The colored ribbons inside the innermost track (blue, pink, and purple) represent homologous mappings between the two genomes, C227-11 and 55989, indicating a high degree of similarity between these genomes. The red lines show the chromosomal positioning of repeat elements, such as insertion sequences and other mobile elements, which reveal more heterogeneity between the genomes than is evident from mapping regions of homology. We compared the sequence of C227-11 with the six O104:H4 strains from Africa, the previously released sequence data from outbreak-linked isolates, the available reference-genome sequences for the outbreak isolate TY2482, and the African enteroaggregative E.
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