In Panel A, the eight circular bands represent the tracks for the different enteroaggregative E. Coli (EAEC) O104:H4 isolates sequenced in our study (from inner to outer tracks: 55989, C227-11, C734-09, C35-10, C682-09, C760-09, C754-09, and C777-09). These different bands represent coverage plots mapped against the reference genome TY2482, with blue or green indicating a high degree of coverage, yellow moderate coverage, and orange or red little or no coverage. The C227-11 coverage plot indicates near-complete sequence identity with the TY2482 genome. The annotations on the outside of the chromosome plot highlight positions of known virulence-factor genes and genes previously characterized on the AA plasmid in the E. Coli O42 strain.
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Coli isolates. The pESBL C227-11 plasmid was not identified in most of the O104:H4 genomes sequenced, suggesting that this plasmid might be a recent acquisition by some strains or, alternatively, might be relatively unstable and consequently lost by numerous strains. The intermediate-size plasmid pAA C227-11 ( ) is approximately 75,000 bp and resembles the pAA plasmid identified in enteroaggregative E.
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The current isolate diverges from common enteroaggregative E. Coli isolates in the number and nature of its SPATE proteases. Most diarrheagenic E. Coli strains encode a single SPATE, and enteroaggregative E. Coli strains ordinarily encode two SPATEs; the presence of three SPATE-encoding genes in the outbreak strain is unusual. One of these SPATEs, Pic, is common among enteroaggregative E.
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